4BU4
RIBONUCLEASE T1 COMPLEX WITH 2'GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1998-02-01 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.500, 48.120, 50.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 * - 1.800 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.25300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1HOH |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.974 |
| Data reduction software | CCP4 |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 * | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.122 * | 0.544 * |
| Total number of observations | 57570 * | |
| Number of reflections | 9347 | |
| <I/σ(I)> | 15.09 | 2.51 |
| Completeness [%] | 96.0 | 96 |
| Redundancy | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.2 | Zegers, I., (1998) Nat. Struct. Biol., 5, 280. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 2'GMP | 2 (mM) | |
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 2 (mM) | ||
| 5 | 1 | reservoir | MPD | 55 (%(v/v)) |
