4BU0
Crystal structure of Rad4 BRCT1,2 in complex with a Crb2 phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 84.823, 63.720, 53.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.290 - 1.500 |
| R-factor | 0.1729 |
| Rwork | 0.172 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bmc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.920 | 1.580 |
| High resolution limit [Å] | 1.490 | 1.500 |
| Rmerge | 0.060 | 0.250 |
| Number of reflections | 47068 | |
| <I/σ(I)> | 6.25 | 2.82 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.92 | 3.58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM SODIUM CITRATE PH 5.6, 20% V/V ISOPROPANOL, 20% W/V PEG 4000 |
