4BTP
Structure of the capsid protein P1 of the bacteriophage phi8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 314.351, 314.350, 527.943 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.010 - 3.700 |
| R-factor | 0.24519 |
| Rwork | 0.244 |
| R-free | 0.25836 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.197 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL2Map |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.830 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.170 | 1.000 |
| Number of reflections | 271956 | |
| <I/σ(I)> | 15.1 | 2.1 |
| Completeness [%] | 97.1 | 71.3 |
| Redundancy | 11.1 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 100 MM TRIS PH 8.5, 30MM SODIUM CITRATE AND BETWEEN 34 AND 39% PEG 200 |
