4BSX
Crystal Structure of the N terminal domain of TRIF (TIR-domain- containing adapter-inducing interferon-beta)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1070, 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.307, 49.331, 70.142 |
| Unit cell angles | 88.66, 77.63, 72.22 |
Refinement procedure
| Resolution | 47.084 - 2.229 |
| R-factor | 0.1942 |
| Rwork | 0.191 |
| R-free | 0.23930 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.358 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX (AUTOSOLVE) |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.8.1_1168)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.440 | 2.350 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.100 | 0.370 |
| Number of reflections | 27104 | |
| <I/σ(I)> | 6.72 | 1.9 |
| Completeness [%] | 93.7 | 71.6 |
| Redundancy | 3.83 | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M BIS-TRIS PH 6.6, 150MM NACL, 28% PEG 3350 |
