4BSS
Structure of the ectodomain of LGR5 in complex with R-spondin-1 (Fu1Fu2) in P21 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.362, 111.298, 130.611 |
| Unit cell angles | 90.00, 109.19, 90.00 |
Refinement procedure
| Resolution | 28.902 - 3.200 |
| R-factor | 0.2487 |
| Rwork | 0.246 |
| R-free | 0.29010 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.166 |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.900 |
| High resolution limit [Å] | 3.200 |
| Rmerge | 0.080 |
| Number of reflections | 52333 |
| <I/σ(I)> | 7.39 |
| Completeness [%] | 97.4 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
