4BSR

Structure of the ectodomain of LGR5 in complex with R-spondin-1 (Fu1Fu2) in P22121 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 2 21 21
Unit cell lengths	85.175, 143.779, 167.338
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.074 - 3.200
R-factor	0.2322
Rwork	0.231
R-free	0.25760
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.003
RMSD bond angle	0.912
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.900
High resolution limit [Å]	3.200
Rmerge	0.150
Number of reflections	34548
<I/σ(I)>	7.2
Completeness [%]	99.7
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1