4BSN

Crystal structure of the Nuclear Export Receptor CRM1 (exportin-1) lacking the C-terminal helical extension at 4.1A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	C 2 2 21
Unit cell lengths	146.960, 246.750, 106.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.049 - 4.100
R-factor	0.2759
Rwork	0.275
R-free	0.29790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gb8
RMSD bond length	0.002
RMSD bond angle	0.612
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8_1056))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	4.350
High resolution limit [Å]	4.100	4.100
Rmerge	0.120	0.500
Number of reflections	15484
<I/σ(I)>	8	2.6
Completeness [%]	99.5	94.5
Redundancy	4.9	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		BY MIXING EQUAL VOLUMES OF PROTEIN (8-10 MG/ML) AND RESERVOIR SOLUTION 0.8 M SODIUM ACETATE, 50 MM TRIS PH 7.5