4BSI
H7N3 Avian Influenza Virus Haemagglutinin in Complex with Avian Receptor Analogue 3'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 116.365, 116.365, 297.213 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.880 - 2.620 |
| R-factor | 0.21734 |
| Rwork | 0.215 |
| R-free | 0.26902 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ti8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.124 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.880 | 2.750 |
| High resolution limit [Å] | 2.620 | 2.620 |
| Rmerge | 0.100 | 0.640 |
| Number of reflections | 23708 | |
| <I/σ(I)> | 13.83 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 291 K, 0.1 M PIPES PH 7.0, 2.2 M AMMONIUM SULFATE, 1% PEG 400 |
