4BSE
Human H7N9 Influenza Virus Haemagglutinin in Complex with Human Receptor Analogue LSTc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 115.890, 115.890, 296.330 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.580 - 2.550 |
| R-factor | 0.23508 |
| Rwork | 0.233 |
| R-free | 0.27891 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ti8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.109 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.550 | 2.690 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.090 | 0.830 |
| Number of reflections | 25386 | |
| <I/σ(I)> | 17.23 | 2.86 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.3 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 291 K, 0.1 M PIPES PH 7.0, 2.2 M AMMONIUM SULFATE, 1% PEG 400 |
