4BSC
Human H7N9 Influenza Virus Haemagglutinin (with Asn-133 Glycosylation) in Complex with Human Receptor Analogue 6'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 116.110, 116.110, 296.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.060 - 2.550 |
| R-factor | 0.22247 |
| Rwork | 0.220 |
| R-free | 0.27988 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ti8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.104 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.050 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.070 | 0.890 |
| Number of reflections | 25430 | |
| <I/σ(I)> | 17.19 | 3 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291 | 291 K, 0.1 M PIPES PH 7.0, 2.2 M AMMONIUM SULFATE, 1% PEG 400 |
