4BRU
Crystal structure of the yeast Dhh1-Edc3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-09-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 105.831, 105.831, 124.648 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.704 - 3.245 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s2m |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.790 |
| Data reduction software | XDS (INTEGRATE) |
| Data scaling software | XDS (CORRECT) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.700 | 3.360 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.090 | 1.240 |
| Number of reflections | 11718 | |
| <I/σ(I)> | 23.5 | 2.4 |
| Completeness [%] | 99.7 | 98.1 |
| Redundancy | 17.3 | 16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM MES PH 6.0, 10% MPD |
