4BRG
Legionella pneumophila NTPDase1 crystal form II (closed) in complex with MG GMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.186, 86.166, 71.878 |
Unit cell angles | 90.00, 107.02, 90.00 |
Refinement procedure
Resolution | 28.990 - 1.450 |
R-factor | 0.1242 |
Rwork | 0.124 |
R-free | 0.17444 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 1.901 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.000 |
High resolution limit [Å] | 1.450 |
Rmerge | 0.050 |
Number of reflections | 127181 |
<I/σ(I)> | 11 |
Completeness [%] | 99.3 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 100MM NAMES PH 5.5, 15% PEG3350, 10% PEG200 |