4BRC
Legionella pneumophila NTPDase1 crystal form II, closed, Mg AMPNP complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.070, 84.756, 70.837 |
| Unit cell angles | 90.00, 106.48, 90.00 |
Refinement procedure
| Resolution | 28.480 - 1.300 |
| R-factor | 0.13638 |
| Rwork | 0.136 |
| R-free | 0.18559 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.044 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.000 |
| High resolution limit [Å] | 1.300 |
| Rmerge | 0.070 |
| Number of reflections | 173270 |
| <I/σ(I)> | 7.3 |
| Completeness [%] | 99.1 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.4 | 100MM NAMES PH 5.4, 15% PEG3350 |
