4BRA
Legionella pneumophila NTPDase1 crystal form II, closed, Mg AMPPNP complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.448, 85.204, 70.736 |
Unit cell angles | 90.00, 108.34, 90.00 |
Refinement procedure
Resolution | 29.180 - 1.600 |
R-factor | 0.12443 |
Rwork | 0.123 |
R-free | 0.18858 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.021 |
RMSD bond angle | 1.982 |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.000 |
High resolution limit [Å] | 1.600 |
Rmerge | 0.070 |
Number of reflections | 91380 |
<I/σ(I)> | 7.7 |
Completeness [%] | 99.2 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.5 | 100MM NAMES PH 5.5, 15% PEG3350 |