4BR9
Legionella pneumophila NTPDase1 crystal form II, closed, apo
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS3 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.504, 86.198, 72.215 |
| Unit cell angles | 90.00, 107.14, 90.00 |
Refinement procedure
| Resolution | 29.200 - 1.400 |
| R-factor | 0.12798 |
| Rwork | 0.127 |
| R-free | 0.17113 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.957 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.000 |
| High resolution limit [Å] | 1.400 |
| Rmerge | 0.040 |
| Number of reflections | 138132 |
| <I/σ(I)> | 21 |
| Completeness [%] | 96.2 |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 9% PEG3350, 100MM (NH4)2SO4, 100MM NAMES PH 5.5 |
