4BR5

Rat NTPDase2 in complex with Zn AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Detector	DECTRIS PILATUS3 2M
Spacegroup name	P 21 21 21
Unit cell lengths	41.170, 69.130, 164.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.710 - 1.750
R-factor	0.17039
Rwork	0.170
R-free	0.19955
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.853
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.730
High resolution limit [Å]	1.750
Rmerge	0.050
Number of reflections	48382
<I/σ(I)>	15
Completeness [%]	100.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5		100 MM HEPES/NAOH PH 7.3, 2% PEG6000, 3MM NAN3