4BR0

rat NTPDase2 in complex with Ca AMPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	41.170, 69.130, 164.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.710 - 2.050
R-factor	0.17077
Rwork	0.169
R-free	0.21847
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.019
RMSD bond angle	1.987
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	36.700
High resolution limit [Å]	2.050
Rmerge	0.070
Number of reflections	30057
<I/σ(I)>	16
Completeness [%]	100.0
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				100 MM HEPES/NAOH PH 7.3, 2% PEG6000, 3MM NAN3