4BQZ

Rat NTPDase2 in complex with Mg GMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	40.880, 69.760, 163.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.580 - 2.050
R-factor	0.18527
Rwork	0.183
R-free	0.23136
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.019
RMSD bond angle	2.021
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	36.600
High resolution limit [Å]	2.050
Rmerge	0.090
Number of reflections	30277
<I/σ(I)>	7.9
Completeness [%]	100.0
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.3		100 MM HEPES/NAOH PH 7.3, 2% PEG6000, 3MM NAN3