4BQN
Structural insights into WcbI, a novel polysaccharide biosynthesis enzyme. Native protein.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-05 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.920, 68.000, 71.490 |
| Unit cell angles | 63.04, 76.45, 69.95 |
Refinement procedure
| Resolution | 24.630 - 1.380 |
| R-factor | 0.11846 |
| Rwork | 0.117 |
| R-free | 0.15502 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.296 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.150 | 1.420 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.020 | 0.180 |
| Number of reflections | 397472 | |
| <I/σ(I)> | 55.9 | 5.7 |
| Completeness [%] | 92.3 | 59.4 |
| Redundancy | 2.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 10% (W/V) PEG 8000, 75 MM LICL, 75 MM MGCL2, 0.05 M HEPES PH 7.0-8.0. |
