4BQ0
Pseudomonas aeruginosa beta-alanine:pyruvate aminotransferase holoenzyme without divalent cations on dimer-dimer interface
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-06 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 111.950, 192.180, 76.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.370 - 1.770 |
| R-factor | 0.21886 |
| Rwork | 0.216 |
| R-free | 0.27574 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b9b |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.451 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.000 | 1.820 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.130 | 0.790 |
| Number of reflections | 2104915 | |
| <I/σ(I)> | 12.3 | 3.1 |
| Completeness [%] | 99.0 | 98.1 |
| Redundancy | 13.2 | 12.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 5.5 | 10 MG/ML PROTEIN, 50MM SODIUM CHLORIDE, 30 MM PYRUVATE, 100 MM SODIUM CITRATE, 20% W/V PEG 3000, PH 5.5, MICROBATCH CRYSTALLIZATION METHOD |
