4BPI
Mcl-1 bound to alpha beta Puma BH3 peptide 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2011-08-11 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.780, 53.780, 93.965 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.688 - 1.982 |
| R-factor | 0.2183 |
| Rwork | 0.217 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nl9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.203 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.690 | 2.050 |
| High resolution limit [Å] | 1.900 | 1.980 |
| Rmerge | 0.110 | 0.830 |
| Number of reflections | 11279 | |
| <I/σ(I)> | 16.88 | 2.95 |
| Completeness [%] | 99.0 | 92.8 |
| Redundancy | 10.09 | 15.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M HEPES, PH7.5 1M SODIUM ACETATE, 0.05M CADMIUM SULPHATE |
