4BPH

High resolution crystal structure of Bacillus subtilis DltC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-07-08
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 65 2 2
Unit cell lengths	49.207, 49.207, 146.264
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.614 - 1.800
R-factor	0.1696
Rwork	0.167
R-free	0.21730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bpf
RMSD bond length	0.004
RMSD bond angle	0.811
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.930	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.090	1.280
Number of reflections	10436
<I/σ(I)>	27.4	2.4
Completeness [%]	100.0	99.9
Redundancy	19.5	19.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		95 MM SODIUM-CITRATE, 19% PEG 4000, 19% 2-PROPANOL, 8% GLYCEROL, PH 5.6