4BPF
High resolution crystal structure of Bacillus subtilis DltC S36A
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.590, 37.222, 37.676 |
| Unit cell angles | 90.00, 114.79, 90.00 |
Refinement procedure
| Resolution | 17.278 - 1.010 |
| R-factor | 0.1302 |
| Rwork | 0.129 |
| R-free | 0.15600 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.353 |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.070 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmerge | 0.130 | 0.790 |
| Number of reflections | 45688 | |
| <I/σ(I)> | 13.9 | 2.5 |
| Completeness [%] | 96.6 | 94.9 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25% W/V PEG 3350, 100 MM BIS-TRIS PH 5.5 |
