4BPD
Structure determination of an integral membrane kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-09-13 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.870, 93.430, 144.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.035 - 3.300 |
R-factor | 0.2431 |
Rwork | 0.241 |
R-free | 0.28210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ze3 |
RMSD bond length | 0.001 |
RMSD bond angle | 0.357 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.380 | 3.390 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.090 | 0.910 |
Number of reflections | 15336 | |
<I/σ(I)> | 10.6 | 2 |
Completeness [%] | 97.1 | 98 |
Redundancy | 4.2 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | 277 | 3-5 %(V/V) 2-METHYL-2, 4-PENTANEDIOL (MPD), 0.1 M SODIUM CHLORIDE, 0.06 M MAGNESIUM ACETATE, 0.1 M SODIUM CITRATE/HCL PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPIDIC CUBIC PHASE, LCP) METHOD AT 4 DEGREES CELCIUS WITH THE 7.8 MONOACYLGLYCEROL (7.8 MAG) AS THE HOSTING LIPID. |