4BPB

STRUCTURAL INSIGHTS INTO RNA RECOGNITION BY RIG-I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	287
Detector technology	CCD
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	47.618, 76.208, 219.825
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.928 - 2.584
R-factor	0.1978
Rwork	0.195
R-free	0.25540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.735
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.680
High resolution limit [Å]	1.900	2.580
Rmerge	0.080	0.610
Number of reflections	25750
<I/σ(I)>	16.22	1.56
Completeness [%]	99.0	92
Redundancy	5.2	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9		0.1 M BICINE, PH 9.0, 22.5 % POLYETHYLENE GLYCOL 6,000