4BP8
Oligopeptidase B from Trypanosoma brucei - open form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-02 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 124.140, 124.140, 249.140 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.730 - 2.400 |
| R-factor | 0.18625 |
| Rwork | 0.184 |
| R-free | 0.23885 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.721 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.170 | 1.500 |
| Number of reflections | 86984 | |
| <I/σ(I)> | 18.6 | 2 |
| Completeness [%] | 99.3 | 95.6 |
| Redundancy | 34.6 | 22.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 9% (W/V) POLYETHYLENE GLYCOL 6000, 1M LITHIUM CHLORIDE, 100MM BIS-TRIS PROPANE, PH 7.5 |
