4BP0
Crystal structure of the closed form of Pseudomonas aeruginosa SPM-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-11 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.650, 83.550, 282.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.943 - 2.240 |
| R-factor | 0.1533 |
| Rwork | 0.152 |
| R-free | 0.19810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fhx |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.174 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.910 | 2.300 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.080 | 0.760 |
| Number of reflections | 53352 | |
| <I/σ(I)> | 15.7 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | PROTEIN WAS CRYTALLISED FROM 10% W/V POLYETHYLENE GLYCOL 8000, 0.1M HEPES, PH7.5, VAPOR DIFFUSION SITTING DROP, ROOM TEMPERATURE |
