4BOD
Complement regulator acquiring outer surface protein BbCRASP-4 or ErpC from Borrelia burgdorferi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 |
| Unit cell lengths | 71.160, 71.160, 75.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.610 - 3.150 |
| R-factor | 0.14174 |
| Rwork | 0.139 |
| R-free | 0.19560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j38 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.412 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.850 | 3.320 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.120 | 0.440 |
| Number of reflections | 5659 | |
| <I/σ(I)> | 6.3 | 2.3 |
| Completeness [%] | 86.3 | 83.4 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 HEPES PH 7.5, 10% ISOPROPANOL, 20% PEG 6000 |
