4BMT
Crystal Structure of Ribonucleotide Reductase di-iron NrdF from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-04-15 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.030, 49.290, 98.510 |
| Unit cell angles | 90.00, 107.16, 90.00 |
Refinement procedure
| Resolution | 47.110 - 2.100 |
| R-factor | 0.21203 |
| Rwork | 0.209 |
| R-free | 0.26040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bmq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.782 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.100 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.100 | 0.300 |
| Number of reflections | 29742 | |
| <I/σ(I)> | 5.7 | 2.7 |
| Completeness [%] | 88.7 | 84.9 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2 M MG-FORMATE, 20% (W/V) PEG 3350, AND 0.1 M HEPES, PH 7.5 |
