4BMQ
Crystal Structure of Ribonucleotide Reductase apo-NrdF from Bacillus cereus (space group C2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-04 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 121.020, 68.200, 87.100 |
| Unit cell angles | 90.00, 106.01, 90.00 |
Refinement procedure
| Resolution | 33.730 - 2.200 |
| R-factor | 0.17906 |
| Rwork | 0.176 |
| R-free | 0.24332 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dhz |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.006 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.200 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.350 |
| Number of reflections | 34588 | |
| <I/σ(I)> | 11.1 | 3.5 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.04 M H3PO4, 16% PEG 8000 AND 20% GLYCEROL |
