4BMH
Crystal structure of SsHAT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 75.885, 75.885, 76.517 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.720 - 1.500 |
| R-factor | 0.14769 |
| Rwork | 0.146 |
| R-free | 0.17457 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.255 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.6.0086) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.720 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.090 | 0.500 |
| Number of reflections | 40822 | |
| <I/σ(I)> | 20.8 | 4.9 |
| Completeness [%] | 99.2 | 98.3 |
| Redundancy | 12.3 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M MGCL2, 0.1 M IMIDAZOLE PH8.0 AND 25% PEG3350 |
