4BMD
Crystal structure of S.pombe Rad4 BRCT3,4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 93.030, 93.030, 75.390 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.048 - 2.500 |
| R-factor | 0.1859 |
| Rwork | 0.184 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xnh |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.667 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.050 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.130 | 0.780 |
| Number of reflections | 13128 | |
| <I/σ(I)> | 5.2 | 0.85 |
| Completeness [%] | 98.2 | 99.9 |
| Redundancy | 4.46 | 4.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M MES PH 6.5, 0.2 M NACL, 20% PEG 6000 |
