4BMC
Crystal structure of s.pombe Rad4 BRCT1,2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.670, 39.270, 72.120 |
| Unit cell angles | 90.00, 103.62, 90.00 |
Refinement procedure
| Resolution | 35.639 - 1.982 |
| R-factor | 0.1903 |
| Rwork | 0.188 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xnh |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.730 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.270 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.120 | 0.470 |
| Number of reflections | 13684 | |
| <I/σ(I)> | 4.89 | 1.51 |
| Completeness [%] | 97.1 | 93 |
| Redundancy | 3.26 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M MES PH 6.0, 1M LITHIUM CHLORIDE, 15-20% PEG 6000 |
