4BM2
CRYSTAL STRUCTURE OF MANGANESE PEROXIDASE 4 FROM PLEUROTUS OSTREATUS - CRYSTAL FORM II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-06 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.670, 86.560, 40.030 |
| Unit cell angles | 90.00, 107.64, 90.00 |
Refinement procedure
| Resolution | 43.280 - 1.391 |
| R-factor | 0.2221 |
| Rwork | 0.221 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fmu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.256 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.470 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.150 | 0.580 |
| Number of reflections | 78216 | |
| <I/σ(I)> | 7.5 | 2.2 |
| Completeness [%] | 96.9 | 87.4 |
| Redundancy | 6.4 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M NA-ACETATE AT PH 4.6 & 2.0 M (NH4)2SO4. |
