4BLN
CRYSTAL STRUCTURE OF FUNGAL VERSATILE PEROXIDASE I FROM PLEUROTUS OSTREATUS - CRYSTAL FORM III
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-06 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 43 |
| Unit cell lengths | 94.600, 94.600, 38.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.300 - 1.149 |
| R-factor | 0.1674 |
| Rwork | 0.166 |
| R-free | 0.19150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fmu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.372 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.220 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.080 | 0.850 |
| Number of reflections | 120788 | |
| <I/σ(I)> | 17.6 | 2.1 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 12.2 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20 % PEG 3350 & 0.2 M AMMONIUM CHLORIDE. |
