4BLL

Crystal Structure of Fungal Versatile Peroxidase I from Pleurotus ostreatus - Crystal Form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS
Synchrotron site	SLS
Temperature [K]	100
Collection date	2012-11-06
Spacegroup name	P 43
Unit cell lengths	62.900, 62.900, 99.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.477 - 1.099
R-factor	0.1238
Rwork	0.123
R-free	0.13560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fmu
RMSD bond length	0.011
RMSD bond angle	1.483
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.170
High resolution limit [Å]	1.100	1.100
Rmerge	0.080	0.980
Number of reflections	154452
<I/σ(I)>	18.3	1.6
Completeness [%]	98.9	93.5
Redundancy	11.8	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M NA-ACETATE AT PH 4.5 & 20 % PEG-1000, 0.2 M ZN(OAC)2.