4BKT

von Hippel Lindau protein:ElonginB:ElonginC complex, in complex with (2S,4R)-N-methyl-1-[2-(3-methyl-1,2-oxazol-5-yl)ethanoyl]-4-oxidanyl-pyrrolidine-2-carboxamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-10-18
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 41 2 2
Unit cell lengths	93.180, 93.180, 364.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.590 - 2.350
R-factor	0.192
Rwork	0.190
R-free	0.22750
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	BUSTER (TNT)
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.350	2.350
Rmerge	0.150	1.500
Number of reflections	68245
<I/σ(I)>	14.6	15.87
Completeness [%]	100.0	100
Redundancy	12.9	13.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1M NA CACODYLATE PH 6.0, 0.2 M MG ACETATE, 15% PEG3350, 5 MM DTT