4BKM
Crystal structure of the murine AUM (phosphoglycolate phosphatase) capping domain as a fusion protein with the catalytic core domain of murine chronophin (pyridoxal phosphate phosphatase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-12-14 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.500, 91.960, 105.920 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 41.737 - 2.650 |
| R-factor | 0.2006 |
| Rwork | 0.198 |
| R-free | 0.25640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oyc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.833 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.740 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.120 | 0.820 |
| Number of reflections | 36245 | |
| <I/σ(I)> | 6.6 | 1.4 |
| Completeness [%] | 96.2 | 94.9 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | MOTHER LIQUOR: 15% PEG 3350, 0.2 M MG(NO3)2. PROTEIN BUFFER: 10 MM TRIETHANOLAMINE PH 7.4, 0.1 M NACL, 1 MM MGCL2 PROTEIN CONCENTRATION: 10 MG/ML |
