4BKG
crystal structure of human diSUMO-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION NOVA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-01-24 |
| Detector | OXFORD DIFFRACTION |
| Wavelength(s) | 1.5406 |
| Spacegroup name | H 3 |
| Unit cell lengths | 74.988, 74.988, 33.267 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.760 - 2.110 |
| R-factor | 0.18092 |
| Rwork | 0.179 |
| R-free | 0.23074 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wm3 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.879 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.240 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.060 | 0.440 |
| Number of reflections | 3842 | |
| <I/σ(I)> | 12.5 | 1.9 |
| Completeness [%] | 94.8 | 73.9 |
| Redundancy | 2.8 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.1 M TRIS/HCL PH 8.0, 28% PEG 350MME,0.05% DIOXANE |
