4BKE
Recombinant human serum albumin with palmitic acid. Synthetic cationic antimicrobial peptides bind with their hydrophobic parts to drug site II of human serum albumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU R-AXIS IV |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.366, 39.003, 96.296 |
| Unit cell angles | 90.00, 105.48, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.350 |
| R-factor | 0.20167 |
| Rwork | 0.198 |
| R-free | 0.25434 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e7h |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.125 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.140 | 2.480 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.070 | 0.660 |
| Number of reflections | 27926 | |
| <I/σ(I)> | 9.3 | 2.2 |
| Completeness [%] | 96.5 | 95.2 |
| Redundancy | 5 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 26 % PEG 3350, 50 MM KH2PO4 PH 7.2; 120 MG/ML IN 20 MM KH2PO4 AT PH 7.5 CONTAINING NOMINAL 0.1 MM PALMITIC ACID STREAK SEEDED SEEDING |
