4BK9
Crystal structure of 2-keto-3-deoxy-6-phospho-gluconate aldolase from Zymomonas mobilis ATCC 29191
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER 1-MICROS |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2011-11-16 |
| Detector | BRUKER |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.380, 118.050, 55.910 |
| Unit cell angles | 90.00, 109.51, 90.00 |
Refinement procedure
| Resolution | 7.940 - 2.770 |
| R-factor | 0.13729 |
| Rwork | 0.135 |
| R-free | 0.17206 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fq0 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.729 |
| Data scaling software | XPREP |
| Phasing software | MrBUMP (USING MOLREP) |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.030 | 2.850 |
| High resolution limit [Å] | 2.770 | 2.770 |
| Rmerge | 0.090 | 0.310 |
| Number of reflections | 15242 | |
| <I/σ(I)> | 10.42 | 2.53 |
| Completeness [%] | 94.9 | 94.1 |
| Redundancy | 3.24 | 2.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | MICROBATCH, 1 UL 10 MG/ML PROTEIN PLUS 1 UL 100 MM NAOAC/ACOH PH 4.6, 200 MM (NH4)2SO4, 30% W/V PEG 4000 |
