4BJY
Crystal structure of 3-hydroxybenzoate 6-hydroxylase uncovers lipid- assisted flavoprotein strategy for regioselective aromatic hydroxylation: Platinum derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | ADSC CCD |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 106.610, 106.610, 142.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.520 |
| R-factor | 0.17507 |
| Rwork | 0.173 |
| R-free | 0.20499 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.104 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.8.0033) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.300 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 62993 | |
| <I/σ(I)> | 33.5 | 6.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 23.7 | 24.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 28% PEG 4000, 0.2 M SODIUM ACETATE AND 0.1 M TRIS-HCL (PH 8.5) |
