4BJN
Crystal structure of the flax-rust effector AvrM-A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 116.990, 131.390, 280.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.590 - 2.900 |
| R-factor | 0.2094 |
| Rwork | 0.207 |
| R-free | 0.24890 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.980 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.700 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.060 | 0.700 |
| Number of reflections | 48260 | |
| <I/σ(I)> | 14.3 | 1.6 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 14.3 | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM BIS-TRIS PH 6.5, 50 MM AMMONIUM SULFATE, 30% PENTA-ERYTHRITOL ETHOXYLATE (15/4 EO/OH) |
