4BJ5
Crystal structure of Rif2 in complex with the C-terminal domain of Rap1 (Rap1-RCT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-07-30 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.440, 113.860, 140.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.470 - 3.290 |
| R-factor | 0.1766 |
| Rwork | 0.176 |
| R-free | 0.19290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 4BJ1 AND 3OWT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.500 | 3.300 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmerge | 0.060 | 0.560 |
| Number of reflections | 27170 | |
| <I/σ(I)> | 14.3 | 2.4 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 3.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 22-25% PEG 6000, 100 MM TRIS/HCL PH 8.0, 500 MM LI2SO4 |
