4BJ0
Xyloglucan binding module (CBM4-2 X2-L110F) in complex with branched xyloses
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-13 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.780, 48.790, 44.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.220 - 1.000 |
| R-factor | 0.11486 |
| Rwork | 0.114 |
| R-free | 0.12931 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2y6h |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.238 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.030 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.050 | 0.720 |
| Number of reflections | 80063 | |
| <I/σ(I)> | 25.5 | 2.9 |
| Completeness [%] | 94.7 | 89.3 |
| Redundancy | 11 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25 % W/V PEG 1500, 0.1 M MMT BUFFER PH 5.5, 20 MM SPERMINE, 8 MM XXXG |
