4BIR
RIBONUCLEASE T1: FREE HIS92GLN MUTANT
Experimental procedure
| Source details | ENRAF-NONIUS |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1994-05-05 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.820, 46.530, 41.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| Rwork | 0.187 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rga |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.076 |
| Data reduction software | ROTAVATA |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Phasing software | FROFFT |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.680 |
| Number of reflections | 10404 | |
| <I/σ(I)> | 6.5 | 3 |
| Completeness [%] | 93.9 | 51.3 |
| Redundancy | 4.6 | 2.18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | pH 4.2 |
