4BIA
Crystal structure of SCP2 thiolase from Trypanosoma brucei: The C337A mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-02 |
| Detector | Bruker Platinum 135 |
| Spacegroup name | P 31 |
| Unit cell lengths | 60.128, 60.128, 375.495 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.363 - 2.900 |
| R-factor | 0.2683 |
| Rwork | 0.267 |
| R-free | 0.29560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zbg |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.091 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.720 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.190 | 0.450 |
| Number of reflections | 33721 | |
| <I/σ(I)> | 9.9 | 1.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 100MM BIS TRIS PH5.5, 200MM LITHIUM SULFATE AND 25% PEG3350 |
