4BI8
Crystal structure of the type VI effector-immunity complex Ssp1-Rap1a from Serratia marcescens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-23 |
| Detector | RIGAKU SATURN 944 HG |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.470, 68.470, 92.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.920 - 2.000 |
| R-factor | 0.1775 |
| Rwork | 0.175 |
| R-free | 0.22145 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bi3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.009 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.920 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.110 | 0.590 |
| Number of reflections | 17462 | |
| <I/σ(I)> | 40.9 | 11.4 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 28.2 | 25.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 12.5% PEG1K, 12.5% PEG3350 AND 12.5% MPD. 20% GLYCEROL AS A CRYO-PROTECTANT |
