4BI4
Structure and function of amidase toxin - antitoxin combinations associated with the type VI secretion system of Serratia marcescens.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-13 |
| Detector | RIGAKU SATURN 944 HG |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.790, 64.590, 97.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.930 - 2.210 |
| R-factor | 0.194 |
| Rwork | 0.191 |
| R-free | 0.24604 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bi3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.241 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.930 | 2.260 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.080 | 0.260 |
| Number of reflections | 18726 | |
| <I/σ(I)> | 23 | 8.1 |
| Completeness [%] | 99.9 | 98.5 |
| Redundancy | 7.5 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1M SODIUM CITRATE PH 5.5, 20% PEG 3000 |
