4BI3
Structure and function of amidase toxin - antitoxin combinations associated with the type VI secretion system of Serratia marcescens.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-29 |
| Detector | RIGAKU (SATURN 944 HG) |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.828, 65.260, 97.504 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.230 - 1.850 |
| R-factor | 0.20881 |
| Rwork | 0.207 |
| R-free | 0.23685 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.967 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.750 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.070 | 0.240 |
| Number of reflections | 31054 | |
| <I/σ(I)> | 33.7 | 10.2 |
| Completeness [%] | 99.4 | 96.2 |
| Redundancy | 16 | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 0.2M POTASSIUM SULPHATE, 20% PEG3350, pH 6.4 |
